Biological research activities in the past few years have helped mankind in multiple areas such as medical diagnosis, evolving biotechnology, using forensic biology, virology, and biological systematics. Further, all these developments would not have been possible without the knowledge of DNA sequences. And biological sequencing techniques are the tools that help in acquiring the knowledge of DNA sequences.
Thus, the importance of using biological sequencing techniques increases manifold if you are looking to invest in the field of biological research work. This article sheds healthy insights on all such techniques. So read till the end, and understand how it paces up your research work.
What is DNA sequencing?
DNA of any organism has four bases namely: adenine, cytosine, thymine, and guanine. And DNA sequencing is nothing but a process to determine the mentioned nucleic acid sequence. You can use any kind of biological sequencing technique for DNA sequencing. In return, it gives you the order of the above-mentioned bases in DNA.
Thus, you can say that DNA sequencing helps to find correct DNA sequences using sequential chemical reactions.
Significance of biological sequencing techniques
The major idea behind every biological research is finding a solution for a disease. Biological sequencing techniques give you the DNA sequencing of the organism in light. You can then compare the sequences of healthy and mutated DNA. This will help you to identify the main issue and you can pinpoint the treatment you must give to treat the disease.
DNA sequencing also helps you to categorize the disease-causing microbes, viruses, pathogens. It is because all these microbial species keep mutating, but all have characteristics similar to their parent cell.
Also read: Sequence listing errors to avoid
Process of DNA sequencing
DNA sequencing requires both laboratory processing and later computational examination. After the chemical reaction, the results (data) generated undergo processing in a computer lab. So, accordingly, the sequences of bases (nucleotides) undergo identification.
You must follow the below steps in biological sequencing techniques to identify DNA sequences:
- Preparing the sample
- DNA amplification using PCR amplification
- Purification using PCR
- Pre-preparation of sequencing
- DNA sequencing
Also read: Sequence listing: USPTO standard
What are the different types of biological sequencing techniques
In the above paragraph, we understood the basics of biological sequencing techniques. But the most important thing to know is how to follow these steps. There are multiple types of techniques that researchers use to conduct DNA sequencing.
The basic principle behind this sequencing technique is, a single-stranded DNA is mounted at a pre-decided location using chemicals. Hence, it is Also known as the chemical cleavage method, The, fragments of DNA are then run on a polyacrylamide gel.
Found to be much more accurate than other biological sequencing techniques, it is used in DNA fingerprinting, and other DNA/genetic studies. The use of radiolabeled chemicals makes it a bit harmful, further, the scale of magnification is also poor.
Sanger sequencing runs on the principle of chain termination method and also known as the first-generation DNA sequencing method. The advantages of Sanger sequencing include better read capacity, sequences 700-800bp in a single run. Mostly used for doing the sequencing of bacterial and prokaryotic organisms.
Also read: What is sequence analysis
Whom to choose for best “Biological Sequencing Techniques” services
Biological sequencing techniques are difficult and complex practices to carry out. Further, the next part is to do the sequence listing in your patent application. This requires professional help and that’s why you need to choose us, “The Sequence Listing Company”.
We work with a team of professionals who are familiar with the standards of the sequence listing. The services you get are too easy, and in the minimum price range available nowhere.