Biological research activities in the past few years have helped the mankind in multiple areas such as medical diagnosis, evolving biotechnology, using forensic biology, virology and biological systematics. Further, all these developments would not have been possible without the knowledge of DNA sequences. And biological sequencing techniques are the tools that help in acquiring the knowledge of DNA sequences.
Thus, the importance of using biological sequencing techniques increase manifold if you are looking to invest in the field of biological research work. This article sheds healthy insights on all such techniques. So read till end, and understand how it paces up your research work.
What is DNA sequencing?
DNA of any organism has four bases namely: adenine, cytosine, thymine, and guanine. And DNA sequencing is nothing but a process to determine the mentioned nucleic acid sequence. You can use any kind of biological sequencing techniques for DNA sequencing. In return it gives you the order of above mentioned bases in a DNA.
Thus, you can say that DNA sequencing is used to find correct DNA sequences using sequential chemical reaction.
Also read: Role of biological sequence search
Significance of biological sequencing techniques
Major idea behind every biological research is finding solution for a disease. Biological sequencing techniques give you the DNA sequencing of the organism in light. You can then compare the sequences of healthy and mutated DNA. This will help you to identify the main issue and you can pin point the treatment to be given to treat the disease.
DNA sequencing also helps you to categorize the disease causing microbes, viruses, pathogens. It is because all these microbial species keep mutating, but all have characteristics similar to their parent cell.
Also read: Sequence listing errors to avoid
Process of DNA sequencing
DNA sequencing requires both laboratory processing and later computational examination. After the chemical reaction, the results (data) generated is processed in a computer lab. So, accordingly the sequences of bases (nucleotides) are identified.
Following are the steps followed in biological sequencing techniques to identify to DNA sequences:
- Preparing the sample
- DNA amplification using PCR amplification
- Purification using PCR
- Pre-preparation of sequencing
- DNA sequencing
Also read: Sequence listing: USPTO standard
What are the different types of biological sequencing techniques
In the above paragraph we understood basics of biological sequencing techniques. But the most important thing to know is how all these steps are followed. There are multiple types of techniques used by researchers to conduct DNA sequencing. They are written below:
Maxam-Gilbert Sequencing –
Basic principle behind this sequencing technique is, a single stranded DNA is mounted at a pre decided location using chemicals. Hence, it is Also known as chemical cleavage method, The, fragments of DNA are then run on polyacrylamide gel.
Found to be much more accurate than other biological sequencing techniques, it is used in DNA finger printing, and other DNA/genetic studies. Use of radiolabeled chemicals make it bit harmful, further the scale of magnification is also poor.
Sanger Sequencing –
Sanger sequencing runs on the principle of chain termination method and also known as first generation DNA sequencing method. The advantages of Sanger sequencing includes better read capacity, sequences 700-800bp in a single run. Mostly used for doing the sequencing of bacterial and prokaryotic organisms.
Also read: What is sequence analysis
Whom to choose for best “Biological Sequencing Techniques” services
Biological sequencing techniques are difficult and complex practices to carryout. Further, the next part is to do the sequence listing in your patent application. This requires a professional help and that’s why you need to choose us, “The Sequence Listing Company”.
We work with the team of professionals who are familiar with the standards of sequence listing. The services you get are too easy, and in the minimum price range available nowhere.
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